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Orient Express exhibition officially launched at Arab World Institute (Paris, 3 April 2014)
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We previously reported the development of a new acquired neurogenic HO NHO mouse model, combining spinal cord transection SCI and chemical muscle injury. Pathological mechanisms responsible for ectopic osteogenesis after central neurological damage are still to be elucidated. In this study, we first hypothesized that peripheral nervous system PNS might convey pathological signals from injured spinal cord to muscles in NHO mouse model.
Twenty one C57Bl6 mice were included in this protocol. Bilateral cardiotoxin CTX injection in hamstring muscles was associated with a two-stage surgical procedure, combining thoracic SCI with unilateral peripheral denervation. Volumes of HO Bone Volume, BV were measured 28 days after surgery using micro-computed tomography imaging techniques and histological analyses were made to confirm intramuscular osteogenesis. Volume comparisons were conducted between right and left hind limb of each animal, using a Wilcoxon signed rank test.
Nineteen mice survive the complete SCI and peripheral denervation procedure. Micro-CT measurements showed significantly increased BV in denervated paws 1. Peripheral denervation can be reliably added to spinal cord transection in NHO mouse model. This new experimental design confirms that neuro inflammatory mechanisms induced by central or peripheral nervous system injury plays a key role in triggering ectopic osteogenesis.
This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: All relevant data are within the paper and its Supporting Information files.
There is a growing but controversial literature concerning the link between high-speed rail HSR services and the tourism market. The aim of this paper is to identify this link in the case of two theme parks, namely Disneyland Paris and Futuroscope Parks, both served by an HSR station. Two revealed preference surveys were carried out interviewing tourists at both stations, with the objective of investigating the influence of HSRs on their decision to visit these theme parks. The results significantly diverge.
In the case of Disneyland, tourists declared that the presence of HSR was fundamental in the choice of the destination; they would not have come without it.
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